Nickel-inducible lysis system in Synechocystis sp. PCC 6803.

Title	Nickel-inducible lysis system in Synechocystis sp. PCC 6803.
Authors	Roy Curtiss, Xinyao Liu
Magazine	Proceedings of the National Academy of Sciences of the United Kingdom
Date	12/22/2009
DOI	10.1073/pnas.0911953106
Introduction	A controllable lysis system was engineered in Synechocystis sp. PCC 6803 to enhance lipid extraction for biofuel production. Bacteriophage-derived lysis genes were integrated into the genome and activated by a nickel-inducible signal transduction system. Three strategies were employed: using phage lysis cassettes, expressing endolysin genes while restricting holin genes, and combining lysis genes from different phages. This system significantly induced autolysis in Synechocystis cells with NiSO4 addition, offering an alternative to mechanical or chemical cell breakage and facilitating biofuel recovery from cyanobacteria.
Quote	Roy Curtiss and Xinyao Liu. Nickel-inducible lysis system in Synechocystis sp. PCC 6803.. 2009. DOI: 10.1073/pnas.0911953106
Element	Nickel (Ni)