NA6888 Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate Powder

Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate Powder Description

Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate (EDTA-2Na) crystallizes as a free-flowing white powder with a molecular weight of 372.24 g/mol and decomposition at 248°C. Its hexadentate ligand structure forms stable complexes with divalent and trivalent metal ions (stability constant log K = 16.5 for Fe³⁺), effectively sequestering contaminants in solutions. The compound dissolves readily in water (260 mM maximum solubility, pH 4–6) but is insoluble in organic solvents like ethanol. Pharmacopeial grades exhibit tightly controlled impurities: chloride ≤50 mg/kg, sulfate ≤50 mg/kg, heavy metals (Pb ≤5 mg/kg, Fe ≤5 mg/kg), and undetectable nuclease/protease activities. Moderate hygroscopicity requires storage at ≤65% RH to prevent caking, while thermal gravimetric analysis confirms stability below 200°C.

Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate Powder Applications

Biochemistry & Molecular Biology: Inhibits metal-dependent enzymes (e.g., metalloproteases) at 1–10 mM; preserves nucleic acid integrity in PCR buffers by chelating Mg²⁺. Anticoagulant in blood collection tubes (1.5 mg/mL), preventing coagulation via calcium sequestration.

Food Industry: Antioxidant & Preservative: Prevents lipid oxidation and browning in beverages (≤25 ppm), emulsified foods (≤150 ppm), and thermally processed products (≤250 ppm) as E385.

Industrial Processes: Corrosion inhibitor in electroplating baths (0.5–2 g/L); water treatment agent for scaling ion removal.

Cosmetics: Stabilizes formulations by chelating trace metals; prevents discoloration in shampoos and creams (≤0.5% w/w).

Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate Powder Packaging

Our products are packaged in customized cartons of various sizes based on the material dimensions. Small items are securely packed in PP boxes, while larger items are placed in custom wooden crates. We ensure strict adherence to packaging customization and the use of appropriate cushioning materials to provide optimal protection during transportation.

Packaging: Carton, Wooden Box, or Customized.

Kindly review the packaging details provided for your reference.

Manufacturing Process

1.       Testing Method

(1)    Chemical Composition Analysis - Verified using techniques such as GDMS or XRF to ensure compliance with purity requirements.

(2)    Mechanical Properties Testing - Includes tensile strength, yield strength, and elongation tests to assess material performance.

(3)    Dimensional Inspection - Measures thickness, width, and length to ensure adherence to specified tolerances.

(4)    Surface Quality Inspection - Checks for defects such as scratches, cracks, or inclusions through visual and ultrasonic examination.

(5)    Hardness Testing - Determines material hardness to confirm uniformity and mechanical reliability.

Please refer to the SAM testing procedures for detailed information.

Ethylenediaminetetraacetic Acid, Disodium Salt Dihydrate Powder FAQs

Q1. Can EDTA-2Na be used in food without restrictions?

No. Regulatory limits apply: beverages ≤25 ppm, thermally processed foods ≤250 ppm, and emulsified products ≤150 ppm. Excess use may cause mineral depletion or allergic reactions.

Q2. Why does my EDTA solution appear cloudy?

Cloudiness indicates interaction with hard water ions (Ca²⁺/Mg²⁺). Always use deionized water (resistivity >1 MΩ·cm) and filter through 0.45 μm if needed.

Q3. Is it safe for cell culture applications?

Only endotoxin-free grades (≤0.5 EU/mL) are suitable. Standard grades may contain trace metals that inhibit cell growth; filter-sterilize before use.

Related Information

1.       Common Preparation Methods

EDTA-2Na is synthesized by reacting ethylenediamine with sodium cyanide and formaldehyde under alkaline conditions, followed by hydrolysis to form EDTA. The crude product is acidified to precipitate EDTA, which is then neutralized with sodium carbonate to form the disodium salt. Dihydrate crystals are obtained through recrystallization from water-ethanol mixtures, with activated carbon treatment to remove organic impurities. Final purification involves ion-exchange chromatography to reduce trace metals, vacuum drying at 60°C to achieve ≤0.5% moisture, and milling to uniform particle size (90% <180 μm).