NR6885 Tris Buffered Saline Tablet

Tris Buffered Saline Tablet Description

Tris Buffered Saline Tablets are compressed formulations combining Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) and sodium chloride in precise stoichiometric ratios. Each tablet dissolves completely in deionized water (typically 15 mL–500 mL depending on brand) to generate a buffer solution with a final osmolarity of ~300 mOsm/L and pH 7.6 at 25°C, maintained by Tris-HCl's effective buffering range (pKa = 8.06 at 20°C). The inclusion of NaCl ensures physiological ionic strength (150 mM), while optional additives like Tween 20 (0.05% w/v in TBS-T variants) reduce nonspecific binding in immunoassays. Tablets exhibit low hygroscopicity and decompose above 175°C, enabling stable storage at 15–25°C under anhydrous conditions. Pharmacopeial-grade variants comply with ISO 9001 and ISO 13485 standards, with impurities controlled to ≤0.05% sulfated ash and ≤5 ppm heavy metals (as Pb).

Tris Buffered Saline Tablet Applications

Immunoassays & Diagnostics: Primary wash buffer for ELISA and immunohistochemistry (IHC), minimizing background staining in tissue sections.

Protein Analysis: Standard diluent and wash solution for Western blotting, enhancing antibody-antigen specificity.

Cell Culture: Isotonic rinsing agent for adherent and suspension cells before dissociation or fixation.

Molecular Biology: Base buffer for nucleic acid hybridization and enzyme-linked probes (e.g., gold-silver staining).

Diagnostic Manufacturing: Component in in vitro diagnostic (IVD) kit production under GMP guidelines.

Tris Buffered Saline Tablet Packaging

Our products are packaged in customized cartons of various sizes based on the material dimensions. Small items are securely packed in PP boxes, while larger items are placed in custom wooden crates. We ensure strict adherence to packaging customization and the use of appropriate cushioning materials to provide optimal protection during transportation.

Packaging: Carton, Wooden Box, or Customized.

Kindly review the packaging details provided for your reference.

Manufacturing Process

1.       Testing Method

(1)    Chemical Composition Analysis - Verified using techniques such as GDMS or XRF to ensure compliance with purity requirements.

(2)    Mechanical Properties Testing - Includes tensile strength, yield strength, and elongation tests to assess material performance.

(3)    Dimensional Inspection - Measures thickness, width, and length to ensure adherence to specified tolerances.

(4)    Surface Quality Inspection - Checks for defects such as scratches, cracks, or inclusions through visual and ultrasonic examination.

(5)    Hardness Testing - Determines material hardness to confirm uniformity and mechanical reliability.

Please refer to the SAM testing procedures for detailed information.

Tris Buffered Saline Tablet FAQs

Q1. How do TBS tablets differ from preparing buffer from scratch?

TBS tablets provide standardized, pre-measured formulations that eliminate weighing errors and ensure batch-to-batch consistency. Each tablet dissolves in a specified volume of water (e.g., 500 mL for Takara T9141) to yield 0.05 M Tris-HCl and 0.15 M NaCl at pH 7.6 ± 0.1. This contrasts with manual preparation, where pH adjustment (±0.3 variability) and salt crystallization risks may compromise reproducibility in sensitive assays like ELISA or Western blotting.

Q2. Can TBS tablets be used for cell culture applications?

Only endotoxin-tested grades (e.g., ≤0.5 EU/mL) are suitable for cell culture. Standard tablets may contain trace impurities from manufacturing (e.g., heavy metals ≤5 ppm) that inhibit cell growth. For primary cell rinsing, dissolve tablets in sterile, endotoxin-free water and filter-sterilize (0.22 μm) before use. Avoid tablets with surfactants (e.g., Tween 20) for live-cell work, as detergents disrupt membranes.

Q3. Why does dissolved TBS solution sometimes appear cloudy?

Cloudiness typically indicates incomplete dissolution or water impurities. Hard water (Ca²⁺/Mg²⁺ >100 ppm) reacts with phosphates in tablet binders, forming insoluble precipitates. Always use Type I/II deionized water (resistivity >1 MΩ·cm) and stir for ≥10 minutes at 20–25°C. If cloudiness persists, filter through 0.45 μm before use. Tablets stored >2 years may degrade, releasing free Tris that crystallizes at pH >8.0.

Related Information

1.       Common Preparation Methods

Commercial TBS tablets are manufactured via direct compression of pharmaceutical-grade Tris-HCl, NaCl, and optional surfactants (e.g., Tween 20). Raw materials undergo micronization to achieve particle sizes <50 μm, followed by dry blending in high-shear mixers to ensure homogeneity. The powder mixture is compressed under controlled humidity (RH <30%) at 10–15 kN force to form tablets with hardness ≥50 N, preventing chipping during packaging and transport. Tablets are coated with a moisture barrier film (e.g., hydroxypropyl methylcellulose) to enhance shelf stability. Quality control includes disintegration testing (<3 minutes in 20°C water), pH verification (7.55–7.65 in final solution), and endotoxin screening (<0.5 EU/mL for cell culture grades).