NR6895 D‑(+)-Trehalose Dihydrate Powder

D‑(+)-Trehalose Dihydrate Powder Description

D-(+)-Trehalose Dihydrate Powder crystallizes as orthorhombic prisms with a molecular weight of 378.33 g/mol and dehydration onset at 97°C. The α,α-1,1-glucoside bond configuration provides unique stress-tolerance mechanisms, forming hydrogen bonds with biomolecules to replace water during desiccation. It demonstrates high solubility in water (68.9 g/100mL at 20°C), glycerol (12 g/100mL), and dimethyl sulfoxide (15 g/100mL), but insolubility in hydrocarbons. Thermal analysis reveals endothermic water loss at 97–110°C (14.5% theoretical hydrate), followed by decomposition at 203°C. Pharmaceutical grades maintain strict controls: reducing sugars ≤0.2%, heavy metals (Pb ≤0.5 ppm), endotoxin ≤0.05 EU/mg, and microbial limits (TAMC ≤100 CFU/g).

D‑(+)-Trehalose Dihydrate Powder Applications

Biopharmaceuticals: Stabilizes monoclonal antibodies/vaccines during lyophilization (5–15% w/v); prevents aggregation at -80°C to 40°C.

Food Technology: Humectant in baked goods (≤15% w/w); flavor protector in freeze-dried foods; sucrose substitute (45% relative sweetness).

Diagnostics: Preserves lateral flow antibodies in dry-state immunoassays; extends shelf life >24 months.

Cosmetics: Moisture-retaining agent in serums (≤8%); reduces transepidermal water loss by 30%.

D‑(+)-Trehalose Dihydrate Powder Packaging

Our products are packaged in customized cartons of various sizes based on the material dimensions. Small items are securely packed in PP boxes, while larger items are placed in custom wooden crates. We ensure strict adherence to packaging customization and the use of appropriate cushioning materials to provide optimal protection during transportation.

Packaging: Carton, Wooden Box, or Customized.

Kindly review the packaging details provided for your reference.

Manufacturing Process

1.       Testing Method

(1)    Chemical Composition Analysis - Verified using techniques such as GDMS or XRF to ensure compliance with purity requirements.

(2)    Mechanical Properties Testing - Includes tensile strength, yield strength, and elongation tests to assess material performance.

(3)    Dimensional Inspection - Measures thickness, width, and length to ensure adherence to specified tolerances.

(4)    Surface Quality Inspection - Checks for defects such as scratches, cracks, or inclusions through visual and ultrasonic examination.

(5)    Hardness Testing - Determines material hardness to confirm uniformity and mechanical reliability.

Please refer to the SAM testing procedures for detailed information.

D‑(+)-Trehalose Dihydrate Powder FAQs

Q1. Why does trehalose crystallize in frozen solutions?

Crystallization occurs below -29°C when the concentration exceeds 40% w/v. Add glycerol (10–20%) to depress the freezing point and maintain amorphous stabilization.

Q2. Can it replace sucrose 1:1 in formulations?

No. Use 1.4× trehalose for equivalent sweetness; reduce to 0.8× for lyoprotection due to higher glass transition temperature (Tg 120°C vs. sucrose 70°C).

Q3. How many freeze-thaw cycles can it protect proteins from?

Maintains ≥95% protein activity after 5 cycles at -80°C↔25°C when used at 0.3–0.5 M concentration.

Related Information

1.       Common Preparation Methods

Enzymatic Conversion: Maltooligosaccharides are transformed to trehalose by Thermus aquaticus maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose trehalohydrolase (MTHase) at 40°C, pH 6.0.

Purification: Crude syrup undergoes ultrafiltration, ion-exchange chromatography, and activated carbon decolorization.

Crystallization: Concentrated solution cooled to 4°C yields dihydrate crystals, centrifuged and washed with chilled ethanol.

Drying: Fluid-bed dried at 35°C/20% RH to preserve hydration; milled to 80% particles 50–200 μm.